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dawn helios ii apparatus  (Waters Corporation)


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    Structured Review

    Waters Corporation dawn helios ii apparatus
    Dawn Helios Ii Apparatus, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 99/100, based on 8475 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dawn+helios+ii+apparatus/DAWN/pm41074781-306-11-15
    Average 99 stars, based on 8475 article reviews
    dawn helios ii apparatus - by Bioz Stars, 2026-11
    99/100 stars

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    Binding Assay:

    Article Title: An Integrative Structural Biology Analysis of Von Willebrand Factor Binding and Processing by ADAMTS-13 in Solution.
    Article Snippet: Membranes were exposed to Hyperfilm ECL films (GE Healthcare). .. Binding of vWF-strep-peptide to AD13-MDTCSE225Q was assessed at a 10:1 molar ratio by SEC-MALLS at the joint IBMB/IRB Automated Crystallography Platform, Barcelona Science Park (Catalonia, Spain) as previously described for other proteins.40,41 Briefly, a Dawn Helios II apparatus (Wyatt Technologies) was used, which was coupled to a SEC Superose 6 10/300 column equilibrated in 10 mM HEPES pH 7.4, 150 mM sodium chloride at 25 C. All experiments were performed in triplicate, and the ASTRA 7 software (Wyatt Technologies) was used for data processing and analysis, for which a dn/dc value typical for proteins (0.185 mL/g) was assumed. .. vWF-strep-peptide was labelled with an aminereactive fluorophore (sulfosuccinimidyl-7-amino-4methylcoumarin-3-acetate; Thermo Fisher Scientific) in 10 mM HEPES pH 7.4, 150 mM sodium chloride at tenfold molar excess of reagent for 1 h at room temperature according to the manufacturer’s instructions.

    Software:

    Article Title: An Integrative Structural Biology Analysis of Von Willebrand Factor Binding and Processing by ADAMTS-13 in Solution.
    Article Snippet: Membranes were exposed to Hyperfilm ECL films (GE Healthcare). .. Binding of vWF-strep-peptide to AD13-MDTCSE225Q was assessed at a 10:1 molar ratio by SEC-MALLS at the joint IBMB/IRB Automated Crystallography Platform, Barcelona Science Park (Catalonia, Spain) as previously described for other proteins.40,41 Briefly, a Dawn Helios II apparatus (Wyatt Technologies) was used, which was coupled to a SEC Superose 6 10/300 column equilibrated in 10 mM HEPES pH 7.4, 150 mM sodium chloride at 25 C. All experiments were performed in triplicate, and the ASTRA 7 software (Wyatt Technologies) was used for data processing and analysis, for which a dn/dc value typical for proteins (0.185 mL/g) was assumed. .. vWF-strep-peptide was labelled with an aminereactive fluorophore (sulfosuccinimidyl-7-amino-4methylcoumarin-3-acetate; Thermo Fisher Scientific) in 10 mM HEPES pH 7.4, 150 mM sodium chloride at tenfold molar excess of reagent for 1 h at room temperature according to the manufacturer’s instructions.

    Size-exclusion Chromatography:

    Article Title: Single-chain dimers from de novo immunoglobulins as robust scaffolds for multiple binding loops
    Article Snippet: .. Size-exclusion chromatography coupled to multiple-angle light scattering (SEC-MALS) was performed in a Dawn Helios II apparatus (Wyatt Technologies) coupled to a SEC Superdex 75 Increase 10/3000 column (Cytiva). ..



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    dawn helios ii apparatus  (Waters Corporation)
    99
    Waters Corporation dawn helios ii apparatus
    Dawn Helios Ii Apparatus, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dawn+helios+ii+apparatus/DAWN/pm41074781-306-11-15
    Average 99 stars, based on 1 article reviews
    dawn helios ii apparatus - by Bioz Stars, 2026-11
    99/100 stars
    		  Buy from Supplier

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